What is the best way to culture Staphylococcus aureus? Tellurite and lithium chloride in Baird-Parker agar inhibit the growth of most bacteria, while pyruvate and glycine specifically promote the growth of S. aureus. Tryptic soy
What is the best way to culture Staphylococcus aureus?
Tellurite and lithium chloride in Baird-Parker agar inhibit the growth of most bacteria, while pyruvate and glycine specifically promote the growth of S. aureus. Tryptic soy broth (TSB) and BHI are the preferred media to grow cultures of Staphylococci. Cultures are grown at 37°C with aeration.
What are the identification laboratory methods of Staphylococcus aureus?
Diagnosis is based on performing tests with colonies. Tests for clumping factor, coagulase, hemolysins and thermostable deoxyribonuclease are routinely used to identify S aureus. Commercial latex agglutination tests are available. Identification of S epidermidis is confirmed by commercial biotyping kits.
What Agar is used for Staphylococcus aureus?
Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others.
What is Staphylococcus aureus in microbiology?
Staphylococcus aureus bacteria are pathogens to both man and other mammals. They are gram positive bacteria that are small round in shape (cocci) and occur as clusters appearing like a bunch of grapes on electron microscopy.
Is Staphylococcus aureus a method?
Results and discussion. Coagulase testing is the single most reliable method for identifying Staphylococcus aureus. Coagulase production can be detected using either the slide coagulase test (SCT) or the tube coagulase test (TCT).
What is best antibiotic for Staphylococcus aureus?
The treatment of choice for S. aureus infection is penicillin. In most countries, S. aureus strains have developed a resistance to penicillin due to production of an enzyme by the bacteria called penicillinase.
What are the main causes of Staphylococcus aureus?
These bacteria are spread by having direct contact with an infected person, by using a contaminated object, or by inhaling infected droplets dispersed by sneezing or coughing. Skin infections are common, but the bacteria can spread through the bloodstream and infect distant organs.