What is the protease substrate? Proteases are enzymes that proteolytically cleave peptide bonds and account for around two percent of all human gene products [1]. Substrate specificity of a protease is determined by molecular interactions
What is the protease substrate?
Proteases are enzymes that proteolytically cleave peptide bonds and account for around two percent of all human gene products [1]. Substrate specificity of a protease is determined by molecular interactions at the protein-protein interface of protease and substrate in the binding cleft of the protease.
How to measure protease activity?
To determine the activity in a solid protease sample diluted in enzyme diluent, divide activity in units/mL by the concentration of solid used in this assay originally in mg/mL, which gives activity in terms of units/mg.
What is the purpose of proteases?
The function of proteases is to catalyze the hydrolysis of proteins, which has been exploited for the production of high-value protein hydrolysates from different sources of proteins such as casein, whey, soy protein and fish meat.
What is the end product of protease?
Where enzymes are produced
| Enzyme | Substrate | End-products |
|---|---|---|
| Protease | Protein | Amino acids |
| Lipase | Lipids (fats and oils) | Fatty acids and glycerol |
| Pancreatic amylase | Starch | Maltose |
| Maltase | Maltose | Glucose |
Where is protease found?
Protease enzymes are produced in your stomach, pancreas and small intestine.
What is the unit of protease?
APU: One unit of alkaline protease is defined as that amount of enzyme needed to produce an absorbance at 660nm that corresponds to the absorbance of 1μg of tyrosine per minute under the conditions of the assay (pH 8.0 and 30°C).
What would happen if we didn’t have protease?
Acidity is created through the digestion of protein. Therefore a protease deficiency results in an alkaline excess in the blood. This alkaline environment can cause anxiety and insomnia.