What is a CAP binding site? CAP binds a DNA region upstream from the DNA binding site of RNA Polymerase. CAP activates transcription through protein-protein interactions with the α-subunit of RNA Polymerase. cAMP-CAP is required
What is a CAP binding site?
CAP binds a DNA region upstream from the DNA binding site of RNA Polymerase. CAP activates transcription through protein-protein interactions with the α-subunit of RNA Polymerase. cAMP-CAP is required for transcription activation of the lac operon.
Where is the CAP binding site?
When cAMP binds to CAP, the complex binds to the promoter region of the genes that are needed to use the alternate sugar sources. In these operons, a CAP-binding site is located upstream of the RNA-polymerase-binding site in the promoter.
What do CAP proteins do?
Catabolite activator protein (CAP), also known as cyclic AMP receptor protein (CRP), is activated by cyclic AMP and stimulates synthesis of the enzymes that break down non-glucose food molecules.
Is the CAP site an activator?
The catabolite activator protein (CAP, also known as cAMP receptor protein, CRP) is a transcriptional activator, present as homodimer in solution, each subunit including a ligand-binding domain at the N-terminus and a DNA-binding domain at the C-terminus.
Does CAP bend DNA?
DNA bound by the CAP-cAMP complex is bent by ~90 degrees. This DNA bend, coupled with a protein-protein interaction between CAP and RNA polymerase is thought to be the mechanism by which CAP regluates transcription initiation on the chromosome.
What is the function of 5 CAP?
The 5′ cap is added to the first nucleotide in the transcript during transcription. The cap is a modified guanine (G) nucleotide, and it protects the transcript from being broken down. It also helps the ribosome attach to the mRNA and start reading it to make a protein.
What is CAP How does CAP work biology?
CAP has a characteristic helix-turn-helix structure that allows it to bind to successive major grooves on DNA. This opens up the DNA molecule, allowing RNA polymerase to bind and transcribe the genes involved in lactose catabolism. coli may use other sugars for fuel, but must transcribe new genes to do so.
What is another word for activator?
Activator Synonyms – WordHippo Thesaurus….What is another word for activator?