What is the Lineweaver-Burk equation? The Lineweaver-Burk equation is a linear equation, where 1/V is a linear function of 1/[S] instead of V being a rational function of [S]. The Lineweaver-Burk equation can be readily

## What is the Lineweaver-Burk equation?

The Lineweaver-Burk equation is a linear equation, where 1/V is a linear function of 1/[S] instead of V being a rational function of [S]. The Lineweaver-Burk equation can be readily represented graphically to determine the values of Km and Vmax.

## How is Lineweaver-Burk derived?

A Double-Reciprocal or Lineweaver-Burk Plot. A double-reciprocal plot of enzyme kinetics is generated by plotting 1/V0 as a function 1/[S]. The slope is the KM/Vmax, the intercept on the vertical axis is 1/Vmax, and the intercept on the horizontal axis (more…)

**Why is the Lineweaver-Burk plot important in enzyme kinetics?**

Why is the Lineweaver-Burk plot important in enzyme kinetics? – It reveals the presence of prosthetic groups in enzymes. – It makes it easier to determine Vmax.

**How do you calculate Vmax?**

The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, Vmax….plotting v against v / [S] gives a straight line:

- y intercept = Vmax.
- gradient = -Km.
- x intercept = Vmax / Km.

### What is Vmax equal to?

Vmax is equal to the product of the catalyst rate constant (kcat) and the concentration of the enzyme. The Michaelis-Menten equation can then be rewritten as V= Kcat [Enzyme] [S] / (Km + [S]). Kcat is equal to K2, and it measures the number of substrate molecules “turned over” by enzyme per second.

### How do you solve for Vmax and KM?

v = Vmax – Km x v / [S]

- y intercept = Vmax.
- gradient = -Km.
- x intercept = Vmax / Km.

**What is the unit of KM and Vmax?**

KM is defined as the [S] that results in half-maximal reaction rate. Vmax and KM are the two parameters which define the kinetic behavior of an enzyme as a function of [S]. Vmax is a rate of reaction. It will have units of: or or etc.

**Which is an example of a Lineweaver-Burk plot?**

An example of a Lineweaver-Burk plot. In biochemistry, the Lineweaver–Burk plot (or double reciprocal plot) is a graphical representation of the Lineweaver–Burk equation of enzyme kinetics, described by Hans Lineweaver and Dean Burk in 1934.

#### How is Lineweaver-Burk used in kinetic analysis?

Lineweaver-Burk analysis is one method of linearizing substrate-velocity data so as to determine the kinetic constants Km and Vmax. One creates a secondary, reciprocal plot: 1/velocity vs. 1/ [substrate].

#### Can a double reciprocal Lineweaver – Burk plot be made?

Then a double reciprocal or Lineweaver–Burk plot of 1/V0 against 1/ [S] is made. Reversible enzyme inhibitors can be classified as either competitive or noncompetitive, and can be distinguished via a Lineweaver–Burk plot.

**How is noncompetitive inhibition recognized on a Lineweaver-Burk plot?**

Noncompetitive inhibition can also be recognized on a Lineweaver–Burk plot since it increases the slope of the experimental line, and alters the intercept on the y-axis (since Vmax is decreased), but leaves the intercept on the x-axis unchanged (since Km remains constant). Uses of Lineweaver–Burk Plot