What is Illumina sequencing technology? The Illumina semiconductor sequencing method, also known as 1-channel SBS, couples sequencing by synthesis chemistry with complementary metal-oxide semiconductor (CMOS) technology. This allows for low instrument costs and a small
What is Illumina sequencing technology?
The Illumina semiconductor sequencing method, also known as 1-channel SBS, couples sequencing by synthesis chemistry with complementary metal-oxide semiconductor (CMOS) technology. This allows for low instrument costs and a small instrument footprint, all while maintaining the high data accuracy of SBS.
What are the steps of Illumina sequencing?
Illumina sequencing technology works in three basic steps: amplify, sequence, and analyze. The process begins with purified DNA. The DNA is fragmented and adapters are added that contain segments that act as reference points during amplification, sequencing, and analysis.
What is NGS Slideshare?
Next-generation sequencing (NGS), also known as high- throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies including: Illumina (Solexa) sequencing Roche 454 sequencing SOLiD sequencing Ion torrent: Proton / PGM sequencing NEXT GENERATION SEQUENCERS.
What can we learn from DNA sequencing?
The sequence tells scientists the kind of genetic information that is carried in a particular DNA segment. For example, scientists can use sequence information to determine which stretches of DNA contain genes and which stretches carry regulatory instructions, turning genes on or off.
What are the applications of NGS?
NGS technologies are currently used for whole genome sequencing, investigation of genome diversity, metagenomics, epigenetics, discovery of non-coding RNAs and protein-binding sites, and gene-expression profiling by RNA sequencing (reviewed in refs.
What is the benefit of paired-end sequencing?
Paired-end sequencing allows users to sequence both ends of a fragment and generate high-quality, alignable sequence data. Paired-end sequencing facilitates detection of genomic rearrangements and repetitive sequence elements, as well as gene fusions and novel transcripts.