How do you elute a HA tag? The HA-tagged protein binds to the HA-tag specific monoclonal antibody conjugated on an agarose gel. After washing away residual impurities, bound HA-tag proteins can be eluted off the
How do you elute a HA tag?
The HA-tagged protein binds to the HA-tag specific monoclonal antibody conjugated on an agarose gel. After washing away residual impurities, bound HA-tag proteins can be eluted off the affnity column by high concentration of the HA-tag peptide or by low pH buffer.
Is ha a peptide?
The HA peptide, NH2-YPYDVPDYA-COOH, is a synthetic peptide used to competitively elute fusion proteins containing the influenza hemagglutinin (HA) tag bound to immobilized anti-HA affinity resins.
What is ha in Western blot?
The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It is well characterized and is used extensively as a general antibody epitope tag. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What is anti-ha?
Anti-HA is produced in rabbit using a synthetic peptide corresponding to amino acid residues of the human Influenza hemagglutinin (HA), conjugated to KLH. The antibody is affinity-purified on the immobilized immunizing peptide. Anti-HA antibody is specific for N- or C-terminal HA-tagged fusion proteins.
How many kDa is HA tag?
1.1 kDa
The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3).
How big is an HA tag?
The hemagglutinin (HA) tag is a 9-amino acid long peptide corresponding to residues 98-106 of the human influenza HA molecule, an ~63kDa surface glycoprotein required for the infectivity of the human virus.
What is HA peptide used for?
HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.
Can peptides be used with hyaluronic acid?
Peptides work well in tandem with other ingredients, including vitamin C, niacinamide (but don’t use niacinamide and vitamin C together!), antioxidants, and hyaluronic acids. Using a peptide with an alpha hydroxy acid (AHA) will actually make the peptides work less efficiently. Look at the label.
How big is a HA tag?
What is the function of hemagglutinin?
The hemagglutinin(HA) of influenza virus is a major glycoprotein and plays a crucial role in the early stage of virus infection: HA is responsible for binding of the virus to cell surface receptors, and it mediates liberation of the viral genome into the cytoplasm through membrane fusion.