What is igepal ca-630 used for? General description. IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. What is igepal used for? IGEPAL® CA-630 is a nonionic,
What is igepal ca-630 used for?
General description. IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.
What is igepal used for?
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.
Is igepal the same as NP 40?
Nonidet P-40 and IGEPAL CA-630 are the same, those are merely different brand names. According to the manufacturer, Sigma-Aldrich, the two compounds are “chemically indistinguishable”. Both are octylphenoxy poly(ethyleneoxy)ethanol.
What is Nonidet P40 substitute?
Furthermore in Biochemistry, Nonidet-P40 substitute (br) is a nonionic surfactant used in the isolation of membrane complexes. Nonidet-P40 Br Substitute is an anhydrous liquid produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide.
How do you store igepal?
Storage: Store in tightly closed containers. Store in an area that is dry, well-ventilated, away from ignition sources, away from incompatible materials (see Section 10. Stability and Reactivity).
How do you dilute igepal?
Warm stock bottle of IGEPAL CA-630 to 37°C as it is quite viscous at room temperature. Make a 10% solution by adding 4mL of warmed IGEPAL (dispensed via a 5mL syringe attached to an 18 gauge needle) to 36mL of Milli-Q or Molecular Biology Grade sterile dH20.
What is the purpose of Nonidet P-40 in RIPA buffer?
A poly(ether) macromolecule that is polyethylene glycol in which one of the terminal hydroxy groups has been converted to the corresponding p-octylphenyl ether. A nonionic, non-denaturing detergent, it is used for solubilising membrane proteins during isolation of membrane-protein complexes.
What is Nonidet P 40 used for?
Nonidet P-40 (CHEBI:78708) A poly(ether) macromolecule that is polyethylene glycol in which one of the terminal hydroxy groups has been converted to the corresponding p-octylphenyl ether. A nonionic, non-denaturing detergent, it is used for solubilising membrane proteins during isolation of membrane-protein complexes.
What is the function of the NP-40 detergent in the extraction buffer?
NP-40 lysis buffer The solubilizing agent is NP-40, which can be replaced by other detergents at different concentrations. Since NP-40 is a nonionic detergent, this lysis buffer has a milder effect than RIPA buffer. It can be used when protein functions are to be retained with minimal disruption.
Is igepal flammable?
Flammability Class: WILL BURN. Extinguishing Media: Recommended: dry chemical, carbon dioxide, foam.
How do you make a 10% Igepal solution?
Make a 10% solution by adding 4mL of warmed IGEPAL (dispensed via a 5mL syringe attached to an 18 gauge needle) to 36mL of Milli-Q or Molecular Biology Grade sterile dH20. Vortex extensively until solubilized. Add 2 grams AG501-X8 resin to the 40mL 10% IGEPAL solution. This resin will “deionize” the solution.